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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/09/2007 |
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Data da última atualização: |
18/02/2008 |
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Autoria: |
AMARAL, P. de P. R.; ALVES, P. C. M.; MARTINS, N. F.; SILVA, F. R. da; CAPDEVILLE, G. de; SOUZA JÚNIOR, M. T. |
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Título: |
Identification and characterization of a Resistence Gene Analog (RGA) from the Caricaceae Dumort family. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Revista Brasileira de Fruticultura, Jaboticabal, v. 28, n. 3, p. 458-462, dez. 2006. |
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ISSN: |
0100-2945 |
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Idioma: |
Inglês |
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Conteúdo: |
The majority of cloned resistance (R) genes characterized so far contain a nucleotide-binding site (NBS) and a leucine-rich repeat (LRR) domain, where highly conservrd motifs are found. Resistance genes analogs (RGAs) are genetic markers obtained by a PCR-based strategy using degenerated oligonucleotide primers drawn from these highlyconserved "motifs". This strategy has the advantage of the high degree of structural and amino acid sequence conservation that is observed in R genes. The objective of the present study was to search for RGAs in Carica papaya L. and Vasconcellea cauliflora Jacq. A. DC. Out of three combinations of primers tested, only one resulted in amplification. The amplified product was cloned in pCR2.1TOPO and than sequenced using M13 forward and reserve primers. Forty-eight clones were sequenced from each species. The 96 sequencies generated for each species were cleaned of vector sequences and clustered using CAP3 assembler. From the GENEBANK, one RGA was identified in C. papaya showing a BlastX e-value of 2x10-61 to the gb[AAP45165.1] putative disease resistant protein RGA3 (Solanum bulbocastanum). To the extent of our knowledge this is the first report of a RGA in the Caricaceae Dumort Family. Preliminary structural studies were performed to further characterize this putative NBS-LRR type protein. Efforts to search for other RGAs in papaya should continue, mostly to provide basis for the development of transgenic papaya with resistance to diseases.
Cricaceae; Nucleotide-binding site (NBS); V cauliflora C papaya; Papaya. MenosThe majority of cloned resistance (R) genes characterized so far contain a nucleotide-binding site (NBS) and a leucine-rich repeat (LRR) domain, where highly conservrd motifs are found. Resistance genes analogs (RGAs) are genetic markers obtained by a PCR-based strategy using degenerated oligonucleotide primers drawn from these highlyconserved "motifs". This strategy has the advantage of the high degree of structural and amino acid sequence conservation that is observed in R genes. The objective of the present study was to search for RGAs in Carica papaya L. and Vasconcellea cauliflora Jacq. A. DC. Out of three combinations of primers tested, only one resulted in amplification. The amplified product was cloned in pCR2.1TOPO and than sequenced using M13 forward and reserve primers. Forty-eight clones were sequenced from each species. The 96 sequencies generated for each species were cleaned of vector sequences and clustered using CAP3 assembler. From the GENEBANK, one RGA was identified in C. papaya showing a BlastX e-value of 2x10-61 to the gb[AAP45165.1] putative disease resistant protein RGA3 (Solanum bulbocastanum). To the extent of our knowledge this is the first report of a RGA in the Caricaceae Dumort Family. Preliminary structural studies were performed to further characterize this putative NBS-LRR type protein. Efforts to search for other RGAs in papaya should continue, mostly to provide basis for the development of transgenic papaya with resistance to diseases.
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Palavras-Chave: |
cauliflora; Cricaceae; Nucleotide-binding site (NBS); Papaya. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02300naa a2200241 a 4500
001 1654024
005 2008-02-18
008 2006 bl uuuu u00u1 u #d
022 $a0100-2945
100 1 $aAMARAL, P. de P. R.
245 $aIdentification and characterization of a Resistence Gene Analog (RGA) from the Caricaceae Dumort family.
260 $c2006
520 $aThe majority of cloned resistance (R) genes characterized so far contain a nucleotide-binding site (NBS) and a leucine-rich repeat (LRR) domain, where highly conservrd motifs are found. Resistance genes analogs (RGAs) are genetic markers obtained by a PCR-based strategy using degenerated oligonucleotide primers drawn from these highlyconserved "motifs". This strategy has the advantage of the high degree of structural and amino acid sequence conservation that is observed in R genes. The objective of the present study was to search for RGAs in Carica papaya L. and Vasconcellea cauliflora Jacq. A. DC. Out of three combinations of primers tested, only one resulted in amplification. The amplified product was cloned in pCR2.1TOPO and than sequenced using M13 forward and reserve primers. Forty-eight clones were sequenced from each species. The 96 sequencies generated for each species were cleaned of vector sequences and clustered using CAP3 assembler. From the GENEBANK, one RGA was identified in C. papaya showing a BlastX e-value of 2x10-61 to the gb[AAP45165.1] putative disease resistant protein RGA3 (Solanum bulbocastanum). To the extent of our knowledge this is the first report of a RGA in the Caricaceae Dumort Family. Preliminary structural studies were performed to further characterize this putative NBS-LRR type protein. Efforts to search for other RGAs in papaya should continue, mostly to provide basis for the development of transgenic papaya with resistance to diseases. Cricaceae; Nucleotide-binding site (NBS); V cauliflora C papaya; Papaya.
653 $acauliflora
653 $aCricaceae
653 $aNucleotide-binding site (NBS)
653 $aPapaya
700 1 $aALVES, P. C. M.
700 1 $aMARTINS, N. F.
700 1 $aSILVA, F. R. da
700 1 $aCAPDEVILLE, G. de
700 1 $aSOUZA JÚNIOR, M. T.
773 $tRevista Brasileira de Fruticultura, Jaboticabal$gv. 28, n. 3, p. 458-462, dez. 2006.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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